Svitkina, Tatyana M.; Verkhovsky, Alexander B.; Borisy, Gary G. (2021). CIL:23042, Rattus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0NS0SQ6

Association of plectin with myosin II. Platinum replica of nocodazole and treated REF-52 cell with actin depleted by gelsolin and plectin labeled with immunogold (10 nm). Individual plectin molecules in lamella cross-link myosin filaments and their clusters. Some plectin molecules bind to each other. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 10e from J Cell Biol. 1996 Nov;135(4):991-1007.

• 2021

• Borisy, Gary G.
• Svitkina, Tatyana M.
• Verkhovsky, Alexander B.

Preparation: glutaraldehyde fixed tissue; critical_point dried specimen; tannic acid
Relation to intact cell: platinum replica
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: uranyl salt; shadowing and plating; primary antibody plus labeled secondary antibody; immunogold
Processing history: unprocessed raw data

• Cell Image Library Group ID: 8924

• Rattus

• Fibroblast
• Myosin II complex
• Plectin

• Gelsolin treatment
• Nocodazole treatment
• REF-52

• data
• image

• No linguistic content; Not applicable

Samplenumber: 23042

Doi: https://doi.org/10.6075/J0NS0SQ6

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL23042

Reference

• J Cell Biol. 1996 Nov;135(4):991-1007 https://www.ncbi.nlm.nih.gov/pubmed/?term=8922382

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2022-08-12