Maddox, Paul S.; Hyndman, Francie; Monen, Joost; Oegema, Karen; Desai, Arshad (2021). CIL:28775, Homo sapiens. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0319TJ7

Spinning disk confocal time-lapse imaging of mitosis in asynchronous HeLa cells transfected with control, nontargeting siRNA oligonucleotides, or siRNA oligonucleotides targeting HsKNL2. Top, nontargeting siRNA oligonucleotides; bottom, siRNA oligonucleotides targeting HsKNL2. DIC is shown on the left, and YFP-CENP-A is shown on the right. The control cell on the left begins in metaphase and, over the course of the video, completes mitosis successfully. Paired YFP-CENP-A loci oscillate during metaphase and then segregate to opposite poles during anaphase. In the HsKNL2 knockdown cells, YFP-CENP-A is not seen to localize at any point during the video. Two cells begin in metaphase and leave mitosis during the video. The DIC channel shows that chromosomes do not oscillate as seen in controls and, during anaphase, segregate with aberrant poleward movement in the cell on the left and nominal poleward movement in the cell on the right. The video is ∼75 min long, and the frame is ∼40 µm top to bottom. Images were acquired at 60-s intervals).

• 2021

• Desai, Arshad
• Hyndman, Francie
• Maddox, Paul S.
• Monen, Joost
• Oegema, Karen

Images were acquired via a spinning disk confocal microscope (CSU10; McBain Instruments) mounted on an inverted microscope (TE2000e; Nikon). Strain TH32 coexpressing GFP-histone H2b and GFP–γ-tubulin was imaged using a 60× 1.4 NA plan Apo objective with 1.5× auxiliary magnification and a cooled CCD camera (Orca ER; Hamamatsu) binning 2 × 2. Strain OD31 expressing GFP–KNL-2 was imaged in the same manner without the 1.5× auxiliary magnification.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: spinning disk confocal microscopy; differential interference contrast microscopy
Parameter imaged: fluorescence emission; optical path length gradient
Source of contrast: distribution of a specific protein; boundaries between regions with different refractive index
Visualization methods: EYFP; visualization of contiguous regions
Processing history: unprocessed raw data

• Cell Image Library Group ID: 11164

• Homo sapiens

• CENP-A
• Nuclear chromosome

• Anaphase
• HeLa
• Metaphase
• Mitosis

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 28775

Doi: https://doi.org/10.6075/J0319TJ7

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL28775

Reference

• J Cell Biol. 2007 Mar 12;176(6):757-63. Epub 2007 Mar 5. https://www.ncbi.nlm.nih.gov/pubmed/?term=17339379

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2022-08-12