Thoma, Claudio R.; Matov, Alexandre; Gutbrodt, Katrin L.; Hoerner, Christian R.; Smole, Zlatko; Krek, Wilhelm; Danuser, Gaudenz (2021). CIL:26572, Homo sapiens, epithelial cell, renal cell carcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0J101X8

RCC-4 cell expressing wild-type Von Hippel-Lindau protein (pVHL30) stained with anti-alpha-tubulin (green) and anti-GTP-tubulin (red) antibodies. GTP-tubulin localizes to the microtubule (MT) tip and remnants of GTP-tubulin are found along the entire MT lattice. When pVHL30 is expressed in RCC-4 cells, a higher frequency of GTP caps and GTP remnants is observed relative to RCC-4 control (VHL negative). RCC-4 cells were permeabilized in PEM buffer with glycerol and 0.1% Triton-X100, then incubated with primary antibody hMB11 followed by incubation with secondary antibody. Cells were then fixed in cold methanol for 5 min and stained with the polyclonal anti-tubulin antibody followed by secondary antibody. Stacks of images were acquired with an IX70 Delta Vision Spectris microscope using a 60X NA1.4 DIC oil Plan-Apochromat objective. This stack corresponds to J Cell Biol. 2010. 190: 991-1003--Fig 5F (right 2 panels) and the control (left 2 panels in Fig 5F) is CIL# 26572.

• 2021

• Danuser, Gaudenz
• Gutbrodt, Katrin L.
• Hoerner, Christian R.
• Krek, Wilhelm
• Matov, Alexandre
• Smole, Zlatko
• Thoma, Claudio R.

Preparation: methanol fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 8073

• Homo sapiens

• Cytoskeleton
• Epithelial cell
• Microtubule
• Protein complex
• Renal cell carcinoma

• GTPase activity
• Protein binding
• Protein polymerization
• RCC4
• Structural molecule activity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 26572

Doi: https://doi.org/10.6075/J0J101X8

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL26572

Reference

• J Cell Biol. 2010. 190: 991-1003. https://www.ncbi.nlm.nih.gov/pubmed/?term=20855504

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12