CIL:39176, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard; Wolf, R. (2021). CIL:39176, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0H41QRD
- Description
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Details of the origins of the cytopharyngeal ribbons and the quadrulus associated ribbons. At first view the cytopharyngeal ribbons look like they might arise next to basal bodies but it is clear that the cytopharyngeal ribbons arise only from the filamentous reticulum. The quadrulus associated ribbons appear to be analogous to the postciliary microtubules of the somatic ciliature that they curve posteriorly from the quadrulus. However quadrulus associated ribbons do pass very close or even touch the ends of the cytopharyngeal ribbons without becoming part of these latter ribbons. Discoidal vesicles bind to the anterior sides (observer’s left) of the cytopharyngeal ribbons and the smaller set of ribbons lies at its posterior side against the cytopharyngeal membrane. The tubular fingers of the alveoli pass between the cytopharyngeal ribbons next to the nodes of the filamentous reticulum from which the cytopharyngeal ribbons originate. Finally a row of parasomal sacs is seen along the dorsal edge of the quadrulus from which coated vesicles form. TEM taken on 5/23/73 by R. Wolf with Hitachi HU11A operating at 75kV. Neg. 16,250X.
- Date Issued
- 2021
- Researchers
- Methods
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Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation - Series
- Scientific Name
- Anatomy
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 39176
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39176
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12