Allen, Richard (2021). CIL:36762, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J008648Q

Cross section of a cilium showing the 9 + 2 axoneme. Two dynein arms, inner and outer, of heterogeneous appearance arise from the complete microtubule of each doublet. Spokes extend from the doublets toward the central pair. Other densities extend from the central pair and between some of the outer doublets and the membrane. A heavy glycocalyx (polysaccharide coat) is found on the outside of the ciliary membrane as well as the plasma membrane. TEM taken on 2/3/69 by R. Allen with Philips 300 operating at 60kV. Neg. 47,100X. Bar = 0.1µm.

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:38898) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Cell Image Library Group ID: 9864

• Paramecium caudatum

• Cell by organism
• Ciliated Protist
• Cilium
• Eukaryotic cell
• Eukaryotic Protist
• Glycocalyx

• Ciliary or flagellar motility
• Plasma membrane organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36762

Doi: https://doi.org/10.6075/J008648Q

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36762

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12