Allen, Richard (2021). CIL:10013, Didinium nasutum, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00Z72DZ

An oblique section shows the transverse aspect of basal bodies/cilia within one pectinelle of one of the two ciliary girdles of Didinium. Two ribbons of microtubules and a short kinetodesmal fiber arise from the proximal margin of each basal body. These may correspond to transverse microtubules and postciliary microtubules of other ciliates. Tips of parasomal sacs are also present. Microtubular ribbons under the alveoli seem to arise from extensions of the postciliary microtubules. TEM taken on 5/9/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:4663) is in the library and available for quantitative analysis.

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: film; Print from negative scanned for Photoshop.
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 3810

• Didinium nasutum

• Bacterial ectosymbiont
• Cell cortex
• Ciliary rootlet
• Ciliated Protist
• Cilium
• Eukaryotic cell
• Eukaryotic Protist
• Microtubule basal body

• Carolina Biological Supply Company, NC, U.S.A.
• Ciliary cell motility
• Cytosketal organization
• Detection of symbiotic bacterium
• Microtubule cytoskeleton organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10013

Doi: https://doi.org/10.6075/J00Z72DZ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10013

Other resource

• Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/

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2022-08-12