Porter, Iain M.; McClelland, Sarah E.; Khoudoli, Guennadi A.; Hunter, Christopher J.; Andersen, Jens S.; McAinsh, Andrew D.; Blow, J. Julian; Swedlow, Jason R. (2021). CIL:13374, Homo sapiens. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02R3QDV

Z-series of fluorescence images of a Hela cell at mitotic metaphase stained for DNA (blue), Aurora B (green) and tubulin (red). This image is the control for the Bod-1 depletion experiment illustrated in CIL: 13372. Bod-1 is a protein that associates with a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis.

• 2021

• Andersen, Jens S.
• Blow, J. Julian
• Hunter, Christopher J.
• Khoudoli, Guennadi A.
• McAinsh, Andrew D.
• McClelland, Sarah E.
• Porter, Iain M.
• Swedlow, Jason R.

Cells were fixed with 3.7% paraformaldehyde or in methanol for 2 min at −20°C. Mouse anti–α-tubulin DM1A (Sigma-Aldrich), and mouse anti–Aurora B antibody AIM-1 (BD Biosciences) were used at 1:500. Fluorescently labeled secondary antibodies were all obtained from Jackson ImmunoResearch Laboratories. Image corresponds to Figure 5A from JCB vol. 179 no. 2 187-197.

Preparation: chemically fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: constrained iterative deconvolution

• Cell Image Library Group ID: 11195

• Homo sapiens

• Aurora B
• Chromosome, centromeric region
• Spindle

• HeLa S3
• Mitosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13374

Doi: https://doi.org/10.6075/J02R3QDV

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13374

Reference

• JCB vol. 179 no. 2 187-197 https://www.ncbi.nlm.nih.gov/pubmed/?term=17938248
• Porter IM, McClelland SE, Khoudoli GA, Hunter CJ, Andersen JS, McAinsh AD, Blow JJ, Swedlow JR. Bod1, a novel kinetochore protein required for chromosome biorientation. J Cell Biol. 2007 Oct 22;179(2):187-97. https://doi.org/10.1083/jcb.200704098

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2022-08-12