Inoue, Shinya (2021). CIL:11969, Homo sapiens, erythrocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0M907BH

The movie shows the behaviour of human erythrocytes suspended in a percoll gradient and exposed to centrifugal force in the centrifuge polarizing microscope developed by S. Inoue. Here, the contrast is generated by differential interference contrast (DIC). Erythrocytes are distorted in shape in the centrifugal field, the hemoglobin collecting at the lower part of the cell causing it to bulge. The movie shows the additions of single cells to a stack of cells (rouleaux). Upon joining a rouleaux, the erythrocytes instantly recover their normal undistorted biconcave shape.

• 2021

• Inoue, Shinya

See: J F Hoffman and S Inoue (2006). Directly observed reversible shape changes and hemoglobin stratification during centrifugation of human and Amphiuma red blood cells. Proc Natl Acad Sci USA 103:2971-2976.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Data qualification: Processed

• Homo sapiens

• Erythrocyte

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 11969

Doi: https://doi.org/10.6075/J0M907BH

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11969

Reference

• S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA. https://www.worldscientific.com/doi/pdf/10.1142/9789812790866_fmatter

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2022-08-12