Microscopy product ID: 3993
Wide field brain mosaic of a coronal section of brain from a transgenic mouse engineered to overexpress human alpha synuclein immunolabeled for alpha synuclein (Green) and counterstained with Hoescht 33342 (red) to reveal cell nuclei. The Hoescht staining introduced some non-specific background (red haze).
Full resolution data file
Processed mosaic in .tif format (051903_vol.tif). Processed mosaic has all the tiles assembled and is a projection of the 3 optical sections acquired for each tile. The reconstruction has been rotated from the original 90 degrees CCW and flipped on the horizontal axis to conform with the orientation of a standard brain atlas and other images in this set. The original orientation has been uploaded as well (051903montage.tif) and is available for download upon request to the CCDB.
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- Creation Date
- Microscopy product: 2003-02-18. Experiment: 2003-02-18. Project: 2002-09-01 to 2003-06-30.
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- Technical Details
Product type: MOSAIC. Microscopy type: multiphoton. Instrument: BioRad RTS 2000MP Multiphoton
- Related Publications
Price DL, Chow SK, MacLean NAB, Hakozaki H, Peltier S, Martone ME, Ellisman MH (2006). High-resolution large-scale mosaic imaging using multiphoton microscopy to characterize transgenic mouse models of human neurological disorders. Neuroinformatics, 4(1):65-80. doi: 10.1385/NI:4:1:65 PMID: 16595859
Price DL, Martone ME, Masliah MH, Ellisman MH (2003). High-resolution large-scale 3-D mapping studies of alpha-synuclein immunoreactivity in transgenic mice overexpressing human alpha-synuclein. Society for Neuroscience Abstract 297.10
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Branfman Family Foundation
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