Baccei, Anna; Lancelle, Susan (2021). CIL:12599, Canis lupus familiaris, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WQ03TR

Network of stress fibers in an MDCK cell. This transmission electron micrograph of a flat-embedded MDCK cell was taken 60-70nm from the interface between the cell and the coverslip on which it was grown and fixed. The cells were cultured on Thermanox® coverslips, then chemically fixed with glutaraldehyde and osmium tetroxide before being stained en bloc with uranyl acetate. The cells were infiltrated with Spurr's® resin, placed under a vacuum for fifteen minutes, and then baked at 70° C. The coverslips were peeled away from the hardened resin wafers, which were then cut and thin-sectioned with a diamond knife. The 65 nm sections were placed on grids and post-stained with uranyl acetate and lead citrate. They were imaged using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV. Because the section was taken just inside the plasma membrane, many vesicles can be seen in this image, particularly in the lower left corner. A neighboring cell in the upper right is shown to underlap the main cell in this image. Scale bar = 2µm.

• 2021

• Baccei, Anna
• Lancelle, Susan

Preparation: osmium tetroxide fixed tissue; tissue in epoxy resin embedment; lead citrate
Relation to intact cell: dispersed cells in vitro; sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; glutaraldehyde fixed tissue
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 3704

• Canis lupus familiaris

• Epithelial cell
• Stress fiber
• Vesicle

• MDCK

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12599

Doi: https://doi.org/10.6075/J0WQ03TR

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12599

Creative Commons Attribution 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12