Biais, Nicolas; Ladoux, Benoît; Higashi, Dustin; So, Magdalene; Sheetz, Michael (2021). CIL:34520, Neisseria gonorrhoeae, motile cell, bacteria. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0H130PJ

This video shows a typical N. gonorrhoeae microcolony interacting with the tips of our micropillar force sensing device. The video is speeded 10 times (×10) and the pilars are 3 micron apart center to center. A pulling force exerted on a pillar, such as that imposed by an attached pilus fiber, causes a displacement of the pillar tip. The displacement can be correlated to the force applied on the pillar after proper calibrations. Video corresponds to Video 1 from PLoS Biol. 2008 Apr 15;6(4):e87.

• 2021

• Biais, Nicolas
• Higashi, Dustin
• Ladoux, Benoît
• Sheetz, Michael
• So, Magdalene

All optical microscope images were obtained on conventional inverted microscopes (either Olympus IX 71 or Zeiss Axiovert 100).

Preparation: whole mounted tissue
Relation to intact cell: living tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy; force microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data

• Cell Image Library Group ID: 8217

• Neisseria gonorrhoeae

• Bacteria
• Motile cell
• Type IV pilus

• Retraction force

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 34520

Doi: https://doi.org/10.6075/J0H130PJ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL34520

Reference

• PLoS Biol. 2008 Apr 15;6(4):e87. https://www.ncbi.nlm.nih.gov/pubmed/?term=18416602

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2022-08-12