Braine, Catherine; Rice, Marian (2021). CIL:34561, Drosophila melanogaster, flight muscle cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0VM4C9H

Drosophila melanogaster thoracic indirect flight muscle from a four week-old wild-type animal. A longitudinal section of myocyte is displayed. Sections of parallel myofibrils are visible between densely packed mitochondria.

• 2021

• Braine, Catherine
• Rice, Marian

Tissue was dissected using a freeze-fracture technique, fixed in 2.5% glutaraldehyde in 0.1 M sodium cacadylate buffer, pH 7.4, post-fixed in 2% osmium tetroxide solution, stained in 1% uranyl acetate, and embeddeded in EMbed 812 resin. Sections were post-stained with 2% uranyl acetate and stained with Reynolds's Lead Citrate. Images were obtained using transmission electron microscopy techniques, employing a Phillips CM-100 transmission electron microscope. Images were taken on Kodak EM 4489 film, scanned with a Microtek ScanMaker i900, and post-processed using Adobe Photoshop CS4. This image was taken at a magnification of 11500x.

Preparation: glutaraldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide; uranyl salt; lead salt
Processing history: digitized film
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 8583

• Drosophila melanogaster

• Flight muscle cell
• Mitochondrial crista
• Mitochondrion
• Myofibril

• Flight

• data
• image

• No linguistic content; Not applicable

Samplenumber: 34561

Doi: https://doi.org/10.6075/J0VM4C9H

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL34561

Creative Commons Attribution 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12