Maddox, Paul S.; Hyndman, Francie; Monen, Joost; Oegema, Karen; Desai, Arshad (2021). CIL:28777, Caenorhabditis elegans, embryo. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0BZ64P3

Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo that has been depleted of KNL-2 by RNAi. GFP-histone and GFP-γ-tubulin were imaged (strain TH32). Pronuclear migration is slightly disrupted by a chromatin bridge resulting from failed meiotic segregation of the maternal chromatin. As seen in CeCENP-A depletion (CIL 28776), chromosome condensation is disrupted, chromosomes fail to form a proper metaphase plate, and the spindle poles move away from the chromatin masses prematurely. Anaphase segregation fails, and chromatin masses are left in the middle of the cell. Also note the meiotic defect that results in a defective oocyte pronucleus (left side of image at the beginning of the videos; this is the embryo anterior). The video is ∼16 min long, and the frame is ∼30 µm top to bottom. Images were acquired at 10-s intervals.

• 2021

• Desai, Arshad
• Hyndman, Francie
• Maddox, Paul S.
• Monen, Joost
• Oegema, Karen

Images were acquired via a spinning disk confocal microscope (CSU10; McBain Instruments) mounted on an inverted microscope (TE2000e; Nikon). Strain TH32 coexpressing GFP-histone H2b and GFP–γ-tubulin was imaged using a 60× 1.4 NA plan Apo objective with 1.5× auxiliary magnification and a cooled CCD camera (Orca ER; Hamamatsu) binning 2 × 2. Strain OD31 expressing GFP–KNL-2 was imaged in the same manner without the 1.5× auxiliary magnification.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data

• Cell Image Library Group ID: 11164

• Caenorhabditis elegans

• Chromatin bridge
• Embryo
• Gamma tubulin
• Nuclear chromosome
• Spindle pole

• KNL-2 depletion
• Mitosis

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 28777

Doi: https://doi.org/10.6075/J0BZ64P3

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL28777

Reference

• J Cell Biol. 2007 Mar 12;176(6):757-63. Epub 2007 Mar 5. https://www.ncbi.nlm.nih.gov/pubmed/?term=17339379

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2022-08-12