Huranova, M.; Ivani, I.; Benda, A.; Poser, I.; Brody, Y.; Hof, M.; Shav-Tal, Y.; Neugebauer, K.M.; Stanek, D. (2021). CIL:26588, Homo sapiens, cervical epithelium. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0ZP4512

HeLa cells stably expressing BAC-encoded GFP-labeled snRNP protein U2A' and imaged witb a Leica SP5 confocal microscope using a 60x 1.4NA HCX Plan Apo objective lens. The U2A' is confined to the nucleoplasm and enriched in splicing factor compartments and Cajal Bodies (bright dots). See Fig 1A in Huranova et al. 2010 J Cell Biol 191:75-86.

• 2021

• Benda, A.
• Brody, Y.
• Hof, M.
• Huranova, M.
• Ivani, I.
• Neugebauer, K.M.
• Poser, I.
• Shav-Tal, Y.
• Stanek, D.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: frame averaging
Data qualification: Processed

• Cell Image Library Group ID: 10294

• Homo sapiens

• Cervical epithelium
• Pre-mRNA
• SnRNP
• Spliceosomal complex

• HeLa
• RNA metabolic process
• RNA splicing

• data
• image

• No linguistic content; Not applicable

Samplenumber: 26588

Doi: https://doi.org/10.6075/J0ZP4512

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL26588

Reference

• M Huranova et al. 2010 J Cell Biol 191:75-86 https://doi.org/10.1083/jcb.201004030

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2022-08-12