Ris, Hans (2021). CIL:40703, Cricetulus griseus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02J6B57

High voltage (1MeV) transmission electron microscopy image of a 0.5 micrometer section of a Chinese hamster ovary cell in metaphase containing a longitudinal section of a chromatid pair, and revealing the fiber-like structure. The image was taken with a specimen tilt of 55 degrees. Grouped with it is an image with a tilt of 45 degrees, providing a pair that affords an oblique stereo view of the chromosome.

• 1978-05-23

• 2021

• Ris, Hans

Cells in metaphase were fixed, embedded in plastic, and 0.5 um sections cut and stained with uranyl acetate.
See also:
H. Ris 1981 Steroscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96
H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Preparation: chemically fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 11986

• Cricetulus griseus

• Epithelial cell
• Nuclear chromosome

• CHO
• Chromosome organization
• Mitosis
• Mitotic metaphase

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40703

Doi: https://doi.org/10.6075/J02J6B57

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40703

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2023-08-17