Ris, Hans (2021). CIL:24969, Drosophila melanogaster, fat body cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00P0Z0X

HVEM image of thick section of a polytene chromosome from the fat bodyof 3rd instar larvae of Drosophila melanogaster showing the organization into bands and interbands characteristic of polytene chromosomes.
This image was taken with a tilt angle of -5 degrees. The accompanying grouped image, taken at +5 degrees forms a stereo pair, providing a 3D view of the chromosome.

• 2021

• Ris, Hans

Cells were dissected, fixed with formalin, stained with uranyl acetate, embedded in epoxy resin, and 0.5 micrometer sections prepared
(see Ris H, 1981 Stereoscopic Electron Microscopy of Chromosomes, Meth Cell Biol 22:77-96). Grids were observed using the Madison High Voltage Electron Microscope at 1 MeV.

Preparation: formaldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; tungsten compound
Processing history: unprocessed raw data

• Cell Image Library Group ID: 11167

• Drosophila melanogaster

• Fat body cell
• Polytene chromosome

• Chromosome organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 24969

Doi: https://doi.org/10.6075/J00P0Z0X

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL24969

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12