CIL:40662, Rattus, hippocampal neuron
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- Collection
- Cite This Work
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Korobova, Farida; Svitkina, Tatyana (2021). CIL:40662, Rattus, hippocampal neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RR1X1V
- Description
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Platinum replica depicting the cytoskeletal organization of dendritic spines in extracted 14 DIV neurons. Branched actin filaments (cyan) in the head. Image corresponds to Figure 2a in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel is available as CIL 40660. The uncolorized image is available as CIL 40659. Box 1, 2 (this image), and 3 are CIL 40661, 40662, and 40663, respectively.
- Date Issued
- 2021
- Researchers
- Methods
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Please refer to referenced article for details on cell extraction and processing for electron microscopy.
- Technical Details
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Preparation: detergent permeabilized; glutaraldehyde fixed tissue; critical_point dried specimen
Relation to intact cell: dispersed cells in vitro
Item type: grey scale graphic
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: colorized - Series
- Scientific Name
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 40662
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40662
- Korobova F, Svitkina T. Molecular architecture of synaptic actin cytoskeleton in hippocampal neurons reveals a mechanism of dendritic spine morphogenesis. Mol Biol Cell. 2010 Jan 1;21(1):165-76. https://doi.org/10.1091/mbc.e09-07-0596
- Mol Biol Cell. 2010 Jan 1;21(1):165-76. Epub 2009 Nov 4. https://www.ncbi.nlm.nih.gov/pubmed/?term=19889835
Source data
Reference
- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12