Inoue, Shinya (2021). CIL:11946, Arbacia punctulata, oocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0M61HZ7

The movie shows two sequences that begin with an unfertilized Arbacia (sea urchin) egg in which the contents have been stratified using the centrifuge polarizing microscope (CPM) developed by S. Inoue. The main feature seen with the polarizing microscope is the appearance of a negatively birefringent layer of stacked membranes. Sperm are then introduced. A few seconds after sperm entry, this birefringence disappears as stacked membranes fragment. The egg also becomes lighter and rises.

• 2021

• Inoue, Shinya

See:S Inoue et al. 2001. Centrifuge polarizing microscope II. Sample biological applications. J Microsc 201:357-367.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: polarization microscopy
Parameter imaged: retardance
Source of contrast: form birefringence
Visualization methods: visualization of contiguous regions
Data qualification: Processed

• Arbacia punctulata

• Oocyte

• Fertilization

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 11946

Doi: https://doi.org/10.6075/J0M61HZ7

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11946

Reference

• S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA. https://www.worldscientific.com/doi/pdf/10.1142/9789812790866_fmatter

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2022-08-12