CIL:40368, Sus scrofa domestica, kidney epithelial cell
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- Cite This Work
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Ferenz, Nick; Wadsworth, Patricia (2021). CIL:40368, Sus scrofa domestica, kidney epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0Z60MVV
- Description
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Time lapse series of a porcine kidney LLCPK1 cell stably expressing photoactivable GFP-tubulin. Fluorescence was activated at prophase in a vertical strip perpendicular to the spindle orientation. Microtubules in the activated strip show a variety of motions both toward and away from spindle poles. This contrasts with the exclusively poleward motion of spindle microtubules seen at metaphase (and illustrated in the grouped movie CIL:40266). A movie prepared from the time series is included in this group of images.
See Fig 1A in: NP Ferenz and P Wadsworth 2007. Prophase microtubule arrays undergo flux-like behavior in mammalian cells. Mol Biol Cell 18:3993-4002 - Date Issued
- 2021
- Researchers
- Methods
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Cells were imaged using a Nikon Eclipse TE300 microscope with 100x phase NA 1.4 objective lens and spinning disk confocal head (Perkin Elmer) and images captured with a Hamamatsu Orca ER cooled CCD camera every 2 sec. Photoactivation was achieved with a 5-sec exposure to 413-nm light from an X-Cite 120 lamp.
See also: NP Ferenz and P Wadsworth 2007. Prophase microtubule arrays undergo flux-like behavior in mammalian cells. Mol Biol Cell 18:3993-4002 - Technical Details
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Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Data qualification: Processed - Series
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- No linguistic content; Not applicable
- Identifier
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Samplenumber: 40368
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40368
- Nick P. Ferenz and Patricia Wadsworth (2007). Prophase Microtubule Arrays Undergo Flux-like Behavior in Mammalian Cells Molecular Biology of the Cell, 18:10, 3993-4002. https://doi.org/10.1091/mbc.e07-05-0420
- NP Ferenz and P Wadsworth 2007. Prophase microtubule arrays undergo flux-like behavior in mammalian cells. Mol Biol Cell 18:3993-4002 https://www.ncbi.nlm.nih.gov/pubmed/?term=17671163
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- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2025-01-30