Morselli, Eugenia; Mariño, Guillermo; Bennetzen, Martin v.; Eisenberg, Tobias; Megalou, Evgenia; Schroeder, Sabrina; Cabrera, Sandra; Bénit, Paule; Rustin, Pierre; Criollo, Alfredo; Kepp, Oliver; Galluzzi, Lorenzo; Shen, Shensi; Malik, Shoaib Ahmad; Maiuri, Maria Chiara; Horio, Yoshiyuki (2021). CIL:13903, Caenorhabditis elegans, embryo. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0KK99HF

Representative image indicative of autophagic activity of C. elegans [VC199, sir-2.1(ok434)IV] embryo expressing a full-length plgg-1DsRED::LGG-1 fusion fed with UV-killed Escherichia coli with additional 0.2 mM spermidine. Epifluorescence microscopy was performed with a microscope (AxioImager Z2; Carl Zeiss, Inc.) using a Plan Neofluar 40× objective with a 0.75 NA and a 63× Plan Neofluar objective with an NA of 1.25 in oil at RT using a 540 ± 15–nm band-pass excitation filter and a 575-nm long-pass emission filter. Images were taken with a camera (AxioCam MRc5; Carl Zeiss, Inc.) with Axiovision software (Carl Zeiss, Inc.) without further processing. Image: Figure 3A, bottom right panel, in Morselli et al. J Cell Biol 192: 615-629

• 2021

• Bennetzen, Martin v.
• Bénit, Paule
• Cabrera, Sandra
• Criollo, Alfredo
• Eisenberg, Tobias
• Galluzzi, Lorenzo
• Horio, Yoshiyuki
• Kepp, Oliver
• Maiuri, Maria Chiara
• Malik, Shoaib Ahmad
• Mariño, Guillermo
• Megalou, Evgenia
• Morselli, Eugenia
• Rustin, Pierre
• Schroeder, Sabrina
• Shen, Shensi

Relation to intact cell: whole mounted tissue
Item type: recorded image; charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: DsRed
Data qualification: Raw;intensitiesquantitation

• Cell Image Library Group ID: 5407

• Caenorhabditis elegans

• Embryo

• Autophagy
• VC199

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13903

Doi: https://doi.org/10.6075/J0KK99HF

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13903

Reference

• J Cell Biol 192: 615-629 https://www.ncbi.nlm.nih.gov/pubmed/?term=21339330

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12