Shapiro, Lucy; McAdams, Harley (2021). CIL:40007, Caulobacter crescentus CB15. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09S1R5W

An image of slice 210 from the reconstructed volume of a tomographic data set from Caulobacter crescentus. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

• 2021

• McAdams, Harley
• Shapiro, Lucy

Caulobacter cultures (strain 3724) were cryofixed using a cocktail of 2% Osmium tetroxide, 0.5% uranyl acetate in anhydrous acetone following this time and temperature schedule: -90 Celsius for 72 hours, raised 2 Celsius per hour for 15 hours, held at -60 Celsius for 12 hours, raised 5 Celsius per hour for 12 hours, held at -20 Celsius. Film images were acquired using a JEOL 4000, at a magnification of 30,000X, 400.0 Kv, with the objective aperture set at 2.

Preparation: osmium tetroxide fixed tissue; cryofixed tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: uranyl salt
Processing history: recorded image
Data qualification: Processed

• Cell Image Library Group ID: 11209

• Caulobacter crescentus CB15

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40007

Doi: https://doi.org/10.6075/J09S1R5W

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40007

Previous version

• Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J0Q52PFJ

Creative Commons Attribution 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12