Uhlen, M.; Oksvold, P.; Fagerberg, L.; Lundberg, E.; Jonasson, K.; Forsberg, M.; Zwahlen, M.; Kampf, C.; Wester, K.; Hober, S.; Wernerus, H.; Björling, L.; Ponten, F. (2021). CIL:40500, Homo sapiens, epidermoid carcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0KW5DXN

Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibody
HPA044440 (shown in green). HPA044440 is a polyclonal Anti-NOL8 antibody that stains the nucleus and the nucleoli. The cells are also stained with reference markers in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probes/organelles are used as references; (i) DAPI for the nucleus (blue) and (ii) anti-tubulin antibody as internal control and marker of microtubules and (red).

• 2021

• Björling, L.
• Fagerberg, L.
• Forsberg, M.
• Hober, S.
• Jonasson, K.
• Kampf, C.
• Lundberg, E.
• Oksvold, P.
• Ponten, F.
• Uhlen, M.
• Wernerus, H.
• Wester, K.
• Zwahlen, M.

The use of data and images from this site in publications and presentations is permitted provided that the following conditions are met:

The publication and/or presentation are solely for informational and non-commercial purposes.

The source of the data and/or image is referred to this site (www.proteinatlas.org) and/or the above publication is cited.

Preparation: chemically fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: unprocessed raw data
Data qualification: Raw

• Cell Image Library Group ID: 11226

• Homo sapiens

• Epidermoid carcinoma
• Microtubule cytoskeleton
• Nucleolus
• Nucleus

• A-431
• Cytoskeleton organization
• Nucleus organization
• Organelle organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40500

Doi: https://doi.org/10.6075/J0KW5DXN

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40500

Reference

• Nat Biotechnol. 2010 28(12):1248-50. https://www.ncbi.nlm.nih.gov/pubmed/?term=21139605
• Uhlen M, Oksvold P, Fagerberg L, Lundberg E, Jonasson K, Forsberg M, Zwahlen M, Kampf C, Wester K, Hober S, Wernerus H, Björling L, Ponten F. Towards a knowledge-based Human Protein Atlas. Nat Biotechnol. 2010 Dec;28(12):1248-50. https://doi.org/10.1038/nbt1210-1248

Other resource

• Human Protein Atlas ("PDIA3"): https://www.proteinatlas.org/ENSG00000167004/subcellular

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2022-08-12