Ammar, El-Desouky (2021). CIL:12416, Maize mosaic virus, Zea mays, plant cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0V69HBC

The images in this group show electron micrographs of epidermal and mesophyll cells in thin sections of maize leaves infected with Maize mosaic virus (MMV, Rhabdoviridae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. This tif image is of a mesophyll leaf cell from a maize plant infected with Maize mosaic virus (MMV), and is identical to the labeled jpg file CIL:16309. MMV virions, budding through the inner nuclear membrane, are accumulating between this membrane and the outer nuclear membrane as well as in intracytoplasmic vesicles, sometimes forming crystalline arrays.
Detailed methods: Pieces of leaves were processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Negatives were scanned later to generate these electronic files. See E.-D. Ammar et al. (2005) J. Phytopathology, 153:129-136.

• 2021

• Ammar, El-Desouky

Preparation: tissue in epoxy resin embedment; glutaraldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide; lead salt; uranyl salt
Data qualification: Raw

• Cell Image Library Group ID: 4686

• Maize mosaic virus
• Zea mays

• Host cell cytoplasmic vesicle
• Host cell nuclear membrane
• Plant cell
• Virion

• Viral reproduction

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12416

Doi: https://doi.org/10.6075/J0V69HBC

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12416

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2022-08-12