Raviola, Giuseppina; Lane, Nancy (2021). CIL:11237, Macaca mulatta, Limulus polyphemus, non-pigmented ciliary epithelial cell, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0CV4GJZ

Representative examples of gap junctions from vertebrates (ciliary epithelium from the eye of a Macaca mulatta, upper) and invertebrates (inverted gap junctions from cells of the mid-gut of the horseshoe crab, Limulus polyphemus, lower). Gap junctions in vertebrates consist of closely aggregated 8-9nm particles on the P-face, whereas those in invertebrate tissues have somewhat larger intramembranous particles associated with the E-face in freeze fracture preparations. Figures 104 (upper, by Giuseppina Raviola) and 105 (lower, from Lane, J. Cell Sci. 32: 293-305, 1978,reprinted with permission in Chapter 3 (Junctional Specializations) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.

• 2021

• Lane, Nancy
• Raviola, Giuseppina

Preparation: freeze_fracture/freeze_etch
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Data qualification: Processed

• Cell Image Library Group ID: 11071

• Limulus polyphemus
• Macaca mulatta

• Connexon complex
• Epithelial cell
• Gap junction
• Non-pigmented ciliary epithelial cell
• Plasma membrane

• Cell communication
• Cell-cell junction organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 11237

Doi: https://doi.org/10.6075/J0CV4GJZ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11237

Reference

• Gilleland MJ, Bender ML. Kinetics of alpha-chymotrypsin dimerization. J Biol Chem. 1976 Jan 25;251(2):498-502. PMID: 1397. https://www.ncbi.nlm.nih.gov/pubmed/?term=01397
• Lane (1978) J. Cell Sci. 32:293-305. http://jcs.biologists.org/content/32/1/293.long
• N.J. Lane; Developmental stages in the formation of inverted gap junctions during turnover in the adult horseshoe crab, Limulus. J Cell Sci 1 August 1978; 32 (1): 293–305. https://doi.org/10.1242/jcs.32.1.293

Other resource

• Fawcett, Don W. "The Cell." 2nd ed., W.B. Saunders, 1981, ISBN 0721635849. https://www.ascb.org/career-development/teaching/don-fawcetts-the-cell/

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2022-08-12