CIL:36634, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard; Schroeder, C. (2021). CIL:36634, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RJ4H49
- Description
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Quick-freeze deep-etch image of an unfixed and fractured mitochondrion. The tubular cristae average 50nm in diameter. Each cristae bears a helically arranged double row of particles with the particles spaced at 12 nm along this zipper-like arrangement. The cristae themselves assume a helical shape and the zipper array remains on the outside curve of the helix. In in situ fractures the 2 enclosing membranes lie close together. Crystals of unknown composition are often found in the mitochondria. TEM taken on 2/15/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 40,500X. Bar = 0.1µm. Published in J. Cell Biol. 108:2233-2240, 1989. Adapted with permission. This image is suitable for qualitative analysis. There is a high resolution version of this image in the library (CIL:40503) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Date Issued
- 2021
- Researchers
- Technical Details
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Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data qualification: Processed - Series
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- Anatomy
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 36634
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36634
- Allen RD, Schroeder CC, Fok AK. An investigation of mitochondrial inner membranes by rapid-freeze deep-etch techniques. J Cell Biol. 1989 Jun;108(6):2233-40. https://doi.org/10.1083/jcb.108.6.2233
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- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12