Microscopy product ID: 4098
2D Image
Description | Single tilt image at zero degrees tilt from a tilt series taken through +/- 60 degrees using an intermediate voltage electron microscope of mitochondria isolated from mouse liver treated with both the serine protease inhibitor MG132 and BH3 peptide from Bid. |
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Description | Zip file containing the tilt images after pre alignment using cross correlation in IMOD (RudyH.preali); the original digitized unaligned images were not available. Also included are the other supporting files generated by IMOD. Some may also be used by TxBr. |
Segmentation
Description | Manual segmentation of the individual cristae and inner and outer mitochondrial membranes using Xvoxtrace 2.18. Contours were traced every other section on even numbered planes. |
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Description | Zip file containing the .trace file generated by Xvoxtrace for the manual contours; and the surface objects generaged by Synu (*.synu). Also included is the Viewdata file required to view the surfaced objects using Synuview. Note that these files may now by opened and viewed using Jinx (see CCDB tools). To view the manual traces superimposed on the volume, the reconstruction file must also be downloaded. |
Reconstruction
Description | Computed slice through an electron tomographic volume of mitochondria isolated from mouse liver and treated with both the protease inhibitor MG132 and BH3 peptide from Bid. |
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Description | Zip file containing the reconstruction file in Analyze 7.5 format: RudyH_newsub.img/.hdr. This reconstruction was used to generate the segmentation shown in the "Segmentation" window. (RudyH_vol.zip) |
Animation
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Description | Animation through the computed slices of an electron tomographic volume of mitochondria isolated from mouse liver and treated with both the protease inhibitor MG132 and BH3 peptide from Bid. |
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- Cite This Work
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Yamaguchi, Ryuji; Newmeyer, Don; Lartigue, Lydia; Perkins, Guy A.; Scott, Ray T.; Dixit, Amruta; (2017). Microscopy product ID: 4098. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J01N80ZW
- Date Issued
- 2017
- Research Team Head
- Researchers
- Technical Details
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Male
Product type: SINGLE TILT. Microscopy type: IVEM. Instrument: JEOL 4000EX IVEM
- Series
- Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Related Resources
- Yamaguchi R, Lartigue L, Perkins G, Scott RT, Dixit A, Kushnareva Y, Kuwana T, Ellisman MH, Newmeyer DD (2008). Opa1-mediated cristae opening is Bax/Bak and BH3 dependent, required for apoptosis, and independent of Bak oligomerization. Molecular Cell, 31(4):557-569. PubMed: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2636708; Molecular Cell: https://doi.org/10.1016/j.molcel.2008.07.010
- Microscopy product 4098 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB4098
Primary associated publication
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- License
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Creative Commons Attribution 4.0 International Public License
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- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22