Wittmann, Torsten; Bokoch, Gary M.; Waterman-Storer, Clare M. (2021). CIL:150, Potorous tridactylus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0Z036WD

Differential interference microscopy (DIC) image of PtK1 cell (marsupial kidney epithelial cell expressing dominant-negative Rac1(T17N). Live cells were used to study microtubule dynamics using X-rhodamine tubulin (injected at 1 mg/mL)and EGFP-Rac1(T17N) (expression vector injected into the cell nucleus at 100 ug/mL). Image corresponds to Fig 3 and microtubule dynamics correspond to Video 3 in J Cell Biol, 161:845-851, 2003. Video 3 is contained within the same image group as this figure.

• 2021

• Bokoch, Gary M.
• Waterman-Storer, Clare M.
• Wittmann, Torsten

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
Data qualification: Raw

• Cell Image Library Group ID: 4105

• Potorous tridactylus

• Cell
• Epithelial cell
• Nucleus

• Dominant-negative Rac
• Pt K1 (NBL-3)

• data
• image

• No linguistic content; Not applicable

Samplenumber: 150

Doi: https://doi.org/10.6075/J0Z036WD

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL150

Reference

• J Cell Biol, 161:845-851, 2003. https://www.ncbi.nlm.nih.gov/pubmed/?term=12796474

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2022-08-12