Microscopy product ID: 49
Manual segmentation of axonal and glial components of the Node of Ranvier using Xvoxtrace v 2.9. Different parts of the myelin sheath were segmented as different objects. Objects were surfaced using both Synu and Amira
Full resolution data file
Zip archive containing the trace file node_marge2.trace and the segmented objects in synu (*.synu) and Amira (*.iv) format. Also included are several renderings of the surface model. These segmentations were produced from the mirrored volume file to mimic a complete node. That is, the original reconstruction of a sagittal section through a node was mirrored to produce the appearance of a single complete node.
Single slice through a single tilt tomogram of the Node of Ranvier from mouse sciatic nerve prepared by high pressure freezing and freeze substitution of aldehyde fixed material
Full resolution data file
Serial tomographic reconstruction from 3 serial volumes. The resulting volume was mirrored and merged with the original to from a relatively complete node of Ranvier. The downloadable zip file contains the merged volume in Analyze 7.5 format
Annotated movie traversing the slices of the serial tomogram and identifying the segmented objects in the surface rendering
- Research Team Heads
- Creation Date
- Microscopy product: 2000-09-26. Experiment: 2000-09-26. Project: 1995-08-01 to 2006-06-30.
- Scientific Name
- Technical Details
Product type: SINGLE TILT. Microscopy type: IVEM. Instrument: JEOL 4000
- Related Publications
Sosinsky GE, Deerinck TJ, Greco R, Buitenhuys CH, Bartol TM, Ellisman MH (2005). Development of a model for microphysiological simulations: small nodes of ranvier from peripheral nerves of mice reconstructed by electron tomography. Neuroinformatics, 3(2):133-62. doi: 10.1385/NI:3:2:133 PMID: 15988042
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
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