Microscopy product ID: 6020
2D Image
Description | Single tilt image from a double tilt series of a 0.2 um section of a VeroE6 cultured cells infected with the SARS-CoV imaged with transmission electron microscopy. Dark particles are 10 nm colloidal gold applied to the section to serve as fiducial markers for alignment of the tilt series. |
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Description | Aligned image stack (6020_Suppmovie1.ali) in IMOD format |
Segmentation
Description | 3D surface-rendered reconstructions of viral structures and adjacent cellular features were processed using AMIRA Visualization Package (TSG Europe, Merignac, France) by surface rendering and thresholding. During this process, some volumes were denoised us |
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Description | Zip file containing surfaced segmented objects in Amira format (ccdb6020_amira.surf) and random snapshots of the rendered objects. The segmentation key for this file can be viewed by "View list of segmented objects" in the Search and Display page for the segmentation of this record. |
Reconstruction
Description | Single computed slice through a dual axis tilt electron tomographic reconstruction of freeze-substituted VeroE6 cultured cells infected with the SARS-CoV. |
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Description | Dual axis tilt electron tomographic reconstruction (6020_Suppmovie1.rec) of a freeze-substituted VeroE6 cultured cells infected with the SARS-CoV at 7h p.i. in IMOD format. |
Animation
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Description | Animation (6020.avi) through the computed slices of a dual axis tomographic volume of freeze-substituted VeroE6 culture cells infected with SARS-CoV, showing interconnected double-membrane vesicles and endoplasmic reticulum. |
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- Collection
- Cite This Work
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Knoops, Kèvin; Koster, Abraham J.; Mommaas, A. Mieke; Snijder, Eric J.; Kikkert, Marjolein; van den Worm, Sjoerd H.E.; Zevenhoven-Dobbe, Jessika C.; van der Meer, Yvonne (2017). Microscopy product ID: 6020. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0N879MJ
- Creation Date
- Microscopy product: 2007-04-07. Experiment: 2006-06-22. Project: 2005-02-01 to 2008-07-25.
- Date Issued
- 2017
- Research Team Heads
- Researchers
- Technical Details
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Product type: DOUBLE TILT. Microscopy type: TEM. Instrument: FEI Tecnai 12
Strain: Vervet
- Funding
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Leiden University Medical Center (LUMC, the Netherlands), Council for Chemical Sciences of the Netherlands Organization for Scientific Research (NOW-CW grants 700.52.306 and 700.55.002), European Commission under context of Euro-Asian SARS-DTV Network (SP22-CT-2004-511064)
- Series
- Scientific Name
- Anatomy
- Topic
Formats
View formats within this collection
- Language
- No linguistic content; Not applicable
- Related Resources
- Knoops K, Kikkert M, van den Worm SHE, Zevenhoven-Dobbe JC, van der Meer Y, Koster AJ, Mommaas AM, Snijder EJ (2008). SARS-coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum. PLoS Biol, 6(9):e226. PubMed: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2535663 PLoS Biology. https://doi.org/10.1371/journal.pbio.0060226
- Microscopy product 6020 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB6020
Primary associated publication
Other version
- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22