CIL:7328, Dictyostelium discoideum, cell by organism, eukaryotic cell, amoeboid cell, Eukaryotic Protist
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- Collection
- Cite This Work
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Clarke, Margaret; Maddera, Lucinda (2021). CIL:7328, Dictyostelium discoideum, cell by organism, eukaryotic cell, amoeboid cell, Eukaryotic Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WD3ZK0
- Description
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A movie of phagosome neutralization visualized using FITC-yeast. Amoebae of Dictyostelium discoideum were fed living budding yeast (S. cerevisiae strain TH2-1B) that had been labeled with fluorescein isothiocyanate (FITC, 0.25 mg/ml). The amoebae in phosphate buffer (pH 6.4) were examined 1 ½ hours later using a Zeiss LSM510 laser scanning confocal microscope; frames were collected in a single focal plane at 5-second intervals. The yeast fluoresce brightly in the extracellular medium, but are dim in acidic phagosomes because FITC fluorescence is quenched at acidic pH. At the end of the endocytic pathway, a phagosome is neutralized prior to exocytosis of the indigestible yeast carcass; that process is visualized here. The amoebae are also expressing two other fluorescent markers that are barely visible, mRFP-LimE-delta, which labels actin filaments, and VatM-GFP, a subunit of the V-ATPase. Three hundred frames from the time series were exported as .tif images (available at CIL:7327)and saved as a stack in ImageJ; they are also shown as a movie (.avi) at 10fps. For related experiments and further details, see Clarke et al., PLoS ONE 5:e8585 (2010).
- Date Issued
- 2021
- Researchers
- Technical Details
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Preparation: whole mounted tissue
Relation to intact cell: whole mounted tissue
Item type: fluorescence microscopy; fluoresence quenching
Imaging mode: recorded image; single-spot confocal microscopy
Parameter imaged: single-spot confocal microscopy
Source of contrast: fluorescence microscopy
Visualization methods: Fluorescein (FITC); PA-mRFP; PA-GFP
Processing history: unprocessed raw data
Data qualification: Raw - Series
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- Identifier
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Samplenumber: 7328
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7328
- Clarke M, Maddera L, Harris RL, Silverman PM. F-pili dynamics by live-cell imaging. Proc Natl Acad Sci U S A. 2008 Nov 18;105(46):17978-81. https://doi.org/10.1073/pnas.0806786105
- PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=19004777
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- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12