Cone, Angela; Sosinsky, Gina; Martone, Maryann (2021). CIL:40199, Mus musculus, CNS neuron (sensu Vertebrata), astrocyte of the hippocampus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0VX0GNT

Wide field mosaic of mouse hippocampus immunolabeled for the hemichannel pore protein pannexin 1 (shown here in green), the astrocytic intermediate filament GFAP (colorized blue) and the gap junction protein connexin 43 (colorized red). The section was also counterstained with DAPI (not shown). 176 tiles (16x11 frames) were digitally stitched together to generate a 3D mosaic montage. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

• 2021

• Cone, Angela
• Martone, Maryann
• Sosinsky, Gina

Mouse brains were perfused with 4% paraformaldehyde, and sections at 50 µm on a vibratome. Tissue was immunostained using the Rabbit Cx43 antibody (Sigma) 1:600; Guinea pig GFAP antibody (discontinued) 1:600; and Chicken Panx1 antibody (4515) 1:250, 72hour incubation at 4C. Secondary antibodies used were donkey-anti-rabbit Al647; donkey-anti-Guinea pig-RRX; and donkey-anti-chicken-FITC, 2.5hours incubation at room temperature. Individual images were gathered using an Olympus Fluoview 1000, with an Olympus PlanApo 60X oil, 1.42 NA.

Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody; Alexa Fluor 488; X-Rhodamine; Alexa Fluor 647
Data qualification: Processed

• Cell Image Library Group ID: 16706

• Mus musculus

• Astrocyte of the hippocampus
• CNS neuron (sensu Vertebrata)
• Gap junction
• Intermediate filament

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40199

Doi: https://doi.org/10.6075/J0VX0GNT

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40199

Previous version

• Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J0N29WSH

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2022-08-12