Ris, Hans (2021). CIL:10711, Notophthalmus viridescens, oocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0ZK5FVG

Meiotic newt oocyte nuclei were allowed to disperse in 30 mM KCl/NaCl, centrifuged through 2% formaldehyde onto an EM grid, stained with uranyl acetate, and critical point dried. Grids were examined with the Madison 1MeV TEM at 10 KX. This micrograph, showing transcription on looped lampbrush chromosomes was recorded at a tilt angle of 55 degrees. The image is grouped with one of the same sample area recorded at a 45 degree tilt. A stereo examination of the pair provides an oblique 3D view.

• 2021

• Ris, Hans

Preparation: formaldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: transmission electron microscopy (TEM)
Imaging mode: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 4641

• Notophthalmus viridescens

• Lampbrush chromosomes
• Oocyte

• Transcription

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10711

Doi: https://doi.org/10.6075/J0ZK5FVG

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10711

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2022-08-12