Microscopy product ID: 3410
2D Image
Description | Single tilt image from a tomographic series of a blue green algae grown in low light conditions imaged with intermediate voltage electron microscopy, showing the chloroplasts and thylakoid membranes. Contrast is reversed so that electron dense structures appear bright. |
Embed
Embed URL
Embed Image
Full resolution data file
File Size |
|
File Format |
|
Description | Tar file containing compressed tilt images in suprim format (*.f.Z), the aligned tilt image (*.s.Z) along with the fiducial mark files produced by xFido (phaeo5.fido) |
Animation
Embed
Embed URL
Embed Video
Description | Animation of aligned tilt series of a blue green algae grown in low light conditions imaged with intermediate voltage electron microscopy, showing the chloroplasts and thylakoid membranes. Contrast is reversed so that electron dense structures appear bright. |
Segmentation
Description | Manual segmentation of the chloroplast membrane and thylakoid membranes, including contact sites, using Xvoxtrace 2.9 |
Embed
Embed URL
Embed Image
Full resolution data file
File Size |
|
File Format |
|
Description | Tar file containing Xvoxtrace file with manual contours. |
Reconstruction
Description | Single computed slice through tomographic volume of blue green algae exposed to low light conditions. Reconstruction shows the chloroplast and inner thylakoid membrane. Some electron dense structures that look like lipid bodies are also visible. |
Embed
Embed URL
Embed Image
Full resolution data file
File Size |
|
File Format |
|
Description | Tar file containing compressed volume files in Analyze 7.5 format. Both the .img and .hdr file are included. Multiple versions of the volume are present, each with a different number of z slices, as indicated by the number in the file name. |
Animation
Embed
Embed URL
Embed Video
Description | Animation through computed slices of tomographic reconstruction of a blue green algae cell grown in low light conditions |
Full metadata
- Collection
- Cite This Work
-
Moisan, Tiffany; Sosinsky, Gina; Buitenhuys, Casey; Ellisman, Mark H. (2017). Microscopy product ID: 3410. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0Q81CVV
- Date Issued
- 2017
- Research Team Head
- Researchers
- Technical Details
-
Product type: SINGLE TILT. Microscopy type: IVEM. Instrument: JEOL 4000EX IVEM
Strain: Karsten
- Funding
-
National Aeronautics and Space Administration
- Series
- Scientific Name
- Anatomy
- Topic
Formats
View formats within this collection
- Language
- No linguistic content; Not applicable
- Related Resources
- Moisan TA, Ellisman MH, Buitenhuys CW, Sosinsky GE (2006). Differences in chloroplast ultrastructure of Phaeocystis antarctica in low and high light. Marine Biology, 149(6):1281-1290. https://doi.org/10.1007/s00227-006-0321-5
- Microscopy product 3410 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB3410
Primary associated publication
Other version
- License
-
Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
-
Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
-
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22