Ladinsky, Mark S.; McIntosh, J. Richard; Mastronarde, David N.; Howell, Kathryn E.; Staehelin, L. Andrew (2021). CIL:24490, Rattus norvegicus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09P30T1

This image is from a tomogram through part of a Golgi ribbon from a normal rat kidney cell, generated using high voltage electron microscopy following incubation at 15°C to block transport out of the Golgi complex. Following temperature block, large bulging domains appear on the three trans-most cisternae. The sample was tilted at angles of 1.5° to obtain the series of images in this set, and used to obtain 3-dimensional reconstructions of the Golgi apparatus and contributed to findings reported in Ladinsky et al. (1999) Golgi structure in three dimensions: Functional insights from the normal rat kidney cell. J. Cell Biol. 144 (6) 1135-1149.

• 2021

• Howell, Kathryn E.
• Ladinsky, Mark S.
• Mastronarde, David N.
• McIntosh, J. Richard
• Staehelin, L. Andrew

Cells were grown on 100-mesh gold EM grids, and maintained at 15°C for 4 hours before plunge freezing in liquid nitrogen (BalTec HPM-010), followed by freeze-substitution (1% glutaraldehyde, 0.1% tannic acid in acetone, replaced with 4% osmium tetroxide and 0.01% uranyl acetate), then embedded in Epon-Araldite and sectioned at 250nm (UltraCut-UCT, Leica). Sections were transferred to formvar-coated copper-rhodium slot grids (EMS) and stained with 2% aqueous uranyl acetate and Reynold's lead citrate. Digital images were acquired at a magnification of 15,000X with an FEI Tecnai TF20 transmission EM. For additional details refer to: J. Cell Biol. 144 (6) 1135-1149.

Preparation: tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: uranyl salt; lead salt
Processing history: unprocessed raw data
Data qualification: Raw

• Cell Image Library Group ID: 8574

• Rattus norvegicus

• Epithelial cell
• Golgi apparatus
• Golgi cisterna

• Golgi organization
• Golgi vesicle budding
• NRK-52E

• data
• image

• No linguistic content; Not applicable

Samplenumber: 24490

Doi: https://doi.org/10.6075/J09P30T1

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL24490

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12