Antipa, Gregory (2021). CIL:17452, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0CR5SJ1

A transverse section near the tip of the oral proboscis of Didinium. This micrograph shows the extrusive organelles of the mucocysts adjacent to the plasma membrane, the toxicysts in a circular ring adjacent to the mucocysts, and the denser pexicysts in the central region of the proboscis. A section deeper in the proboscis is found at (CIL:17451). Further details available at Wessenberg, H. and Antipa, G. 1968. Studies on Didinium nasutum. I. Structure and ultrastructure. Protistologica 4:427-447. And details of the operation of this structure is available at Wessenberg, H. and Antipa, G. 1970. Capture and ingestion of Paramecium by Didinium nasutum. J. Protozool. 17:250-270. TEM taken in 1966 by G. Antipa with JEM T6S operating at 50kV. Neg. 6,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 50nm thickness.

• 2021

• Antipa, Gregory

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 11067

• Didinium nasutum

• Cell by organism
• Ciliated Protist
• Cytoplasm
• Eukaryotic cell
• Eukaryotic Protist
• Extrusomes
• Oral apparatus
• Plasma membrane

• Carolina Biological Supply Company, NC, U.S.A.
• Oral apparatus organization
• Plasma membrane organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 17452

Doi: https://doi.org/10.6075/J0CR5SJ1

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL17452

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12