Microscopy product ID: 3864
2D Image
Description | Zero degree tilt image from a tilt series collected using an intermediate voltage electron microscope through +/- 60 degrees of mitochondria isolated from mouse liver. Section thickness was 0.5 um. |
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Description | Zip file containing the raw and pre-aligned (after cross correlation) tilt series in IMOD format (rudyacontrol.st); all accompanying files are included in the zip archive, although the aligned tilt series was not available. |
Segmentation
Description | Segmentation of the cristae, inner and outer membranes was performed by manual tracing in the planes of highest resolution using Xvoxtrace version 2.18 |
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Description | Zip file containing the .trace file generated by Xvoxtrace containing the manual contours (RudyA.trace), the subvolume used for the tracing in Analyze 7.5 format (RudyAtrace.img/.hdr) and the surfaced contours in synu format (*.synu). The Viewdata file used by the program Synuview for assigning color and transparency was not submitted. Note that the Xvoxtrace files and synu files may be viewed using Jinx, available from the CCDB tool download page. |
Reconstruction
Description | Computed slice through an electron tomographic volume of an isolated mitochondrion from mouse liver from the no treatment condition. |
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Description | Zip file (RudyA.zip) containing the reconstruction file in Analyze 7.5 format: RudyA.img/.hdr. The volume resolution was binned down 2X from the digitized images. A subvolume of this reconstruction was generated for manual segmentation. The subvolume along with the trace file is available through the segmentation download. |
Animation
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Description | Animation through a portion of the tomographic volume. The volume was downsampled for ease of viewing. |
Full metadata
- Collection
- Cite This Work
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Yamaguchi, Ryuji; Newmeyer, Don; Lartigue, Lydia; Perkins, Guy A.; Scott, Ray T.; Dixit, Amruta (2017). Microscopy product ID: 3864. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0W66KJ2
- Date Issued
- 2017
- Research Team Head
- Researchers
- Technical Details
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Product type: SINGLE TILT. Microscopy type: IVEM. Instrument: JEOL 4000EX IVEM
- Series
- Scientific Name
- Anatomy
- Topic
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View formats within this collection
- Language
- No linguistic content; Not applicable
- Related Resources
- Yamaguchi R, Lartigue L, Perkins G, Scott RT, Dixit A, Kushnareva Y, Kuwana T, Ellisman MH, Newmeyer DD (2008). Opa1-mediated cristae opening is Bax/Bak and BH3 dependent, required for apoptosis, and independent of Bak oligomerization. Molecular Cell, 31(4):557-569. PubMed: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2636708; Molecular Cell. https://doi.org/10.1016/j.molcel.2008.07.010
- Microscopy product 3864 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB3864
Primary associated publication
Other version
- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22