Ris, Hans (2021). CIL:24971, Drosophila melanogaster, fat body cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GB22ZC

High Voltage TEM image showing the series of bands and interbands characteristic of a polytene chromosome. This image of a whole mount preparation of a fat body cell polytene chromosome from Drosophila melanogaster was taken with a tilt angle of 45 degrees. The accompanying grouped image, taken at 55 degrees forms a stereo pair, providing an oblique 3D view of the chromosome.

• 2021

• Ris, Hans

Fat body cells from 3rd instar larvae of Drosophila melanogaster were dissected, fixed with formalin-acetic acid containing 0.1% Triton-X 100, and squashed on a glass slide. After freezing in liquid nitrogen, the cover slips removed, and the slide thawed and stained with 1% uranyl acetate, rinsed in 50% ethanol, dehydrated and transferred to amyl acetate, and covered with 2% parlodion in amyl acetate. Selected areas of the parlodion film were transferred to formvar coated EM grids, and the parlodion removed by immersion in amyl acetate (see Ris H, 1981 Stereoscopic Electron Microscopy of Chromosomes, Meth Cell Biol 22:77-96). Grids were observed using the Madison High Voltage Electron Microscope at 1 MeV.

Preparation: formaldehyde fixed tissue; acetic acid treatment
Relation to intact cell: squashed preparation
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 11171

• Drosophila melanogaster

• Fat body cell
• Polytene chromosome

• Chromosome organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 24971

Doi: https://doi.org/10.6075/J0GB22ZC

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL24971

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2022-08-12