Svitkina, Tatyana M.; Borisy, Gary G. (2021). CIL:34918. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0JQ0ZNR

Localization of Arp2/3 complex at actin filament branching points. Xenopus keratocytes and fibroblasts were treated with CD (0.2 μM for 30 min or 0.5 μM for 10 min), extracted in the presence of phalloidin, fixed with glutaraldehyde, treated with 33% methanol, and immunostained with p21 antibody followed by 10-nm gold-conjugated secondary antibody. Image corresponds to a single panel from Figure 4 in J Cell Biol. 1999 May 31;145(5):1009-26. All of the panels from Figure 4 are available as CIL:34902-34921.

• 2021

• Borisy, Gary G.
• Svitkina, Tatyana M.

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Preparation: permeabilized tissue; glutaraldehyde fixed tissue; methanol fixed tissue; phalloidin
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: optical path length gradient
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating; primary antibody plus labeled secondary antibody; platinum replica; immuno-gold
Processing history: unprocessed raw data

• Cell Image Library Group ID: 9875

• Actin cytoskeleton
• Arp2/3 protein complex

• Branching of actin filaments
• Cytochalasin D treatment

• data
• image

• No linguistic content; Not applicable

Samplenumber: 34918

Doi: https://doi.org/10.6075/J0JQ0ZNR

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL34918

Reference

• J Cell Biol. 1999 May 31;145(5):1009-26. https://www.ncbi.nlm.nih.gov/pubmed/?term=10352018

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2022-08-12