Allen, Richard (2021). CIL:13158, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0PR7V41

The proximal end of the basal body is sometimes the origin of other single microtubules or bundles of microtubules that extend deeper into the cell's endoplasm. These guide trichocysts, tip first, to the cell surface and spent vacuoles to the cytoproct. Septal pores connect adjacent alveolar sacs. TEM taken on 1/12/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,350X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12070) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Cell Image Library Group ID: 3972

• Paramecium multimicronucleatum

• Cell by organism
• Cell cortex
• Ciliated Protist
• Cortical cytoskeleton
• Cytoplasm
• Eukaryotic cell
• Eukaryotic Protist
• Microtubule basal body
• Trichocyst

• Cortical cytoskeleton organization
• Cytoplasm organization
• Membrane organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13158

Doi: https://doi.org/10.6075/J0PR7V41

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13158

Other resource

• Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/

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2022-08-12