Ivanchenko, Maryna V.; Cicconet, Marcelo; Jandal, Hoor Al; Wu, Xudong; Corey, David P.; Indzhykulian, Artur A. (2022). CIL: 50692, FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles: 2.5_Cell_2_Stereocilia_Segmentation. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WW7H1S

FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.

• 2022

• Cicconet, Marcelo
• Corey, David P.
• Indzhykulian, Artur A.
• Ivanchenko , Maryna V.
• Jandal, Hoor Al
• Wu, Xudong

Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
2.5_Cell_2_Stereocilia_Segmentation is a multipage TIFF file illustrating stereocilia segmentation results (1.95×1.95×10 nm³ voxel size). Same as ‘Towers.am’ file used for MATLAB volume analysis of this cell, but converted to TIFF to allow for its online preview.
This file is a part of Dataset 2, comprised of files related to the Cell #2 of current study. The numbering order of the files reflects the order of operations performed during the analysis.

Imaging mode: Serial Electron Microscopy; Focused Ion Beam Scanning Electron Mictoscopy (FIB-SEM)
Visualization methods: Heavy metal stain (Osmium Tetroxide, Potassium Ferrocyanide, Tannic Acid); Immunogold labeling with 10 nm gold beads

• Cell Image Library Group ID: 20002

• Mus musculus

• Cuticular plate
• Hair cell stereocilia
• Inner ear outer hair cell
• Mitochondria
• Polycystic Kidney and Hepatic Disease 1-Like 1 (PKHD1L1) labeling (gold beads)

• Native auditory Hair Cells at postnatal day 4

• data
• image

• No linguistic content; Not applicable

Samplenumber: 50692

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL50692

Reference

• Nature article: https://www.nature.com/articles/s41467-019-11712-w
• Nature Communications article: https://www.nature.com/articles/s41467-019-11712-w

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2022-11-18