Hoog, Johanna (2021). CIL:12595, Trypanosoma brucei, cell by organism, eukaryotic cell, Eukaryotic Protist, Flagellated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0QV3KMT

The flagellar pocket of a Trypanosoma brucei bloodstream form cell, with short flagellum. The image shows a new flagellum starting to grow from the flagellar pocket, in preparation for the next cell division. Cells were high pressure frozen and freeze substituted (1hr freeze substitution in 2% uranyl acetate in acetone and 8% methanol). An 80nm thick section was stained for 5 minutes with uranyl acetate, one minute with lead citrate and viewed using a Philips CM100 transmission electron microscope. The image was captured with an Advantage HS-B camera (2000 x 2000 pixels; AMT, Danvers, MA, USA). Contrast adjusted using Photoshop. Magnification indicated by scale bar.

• 2021

• Hoog, Johanna

Preparation: microtome-sectioned tissue; freeze substitution
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; electron bombardment CCD (EBCCD)
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt
Processing history: recorded image; electron bombardment CCD (EBCCD); Contrast enhanced in Photoshop.
Data qualification: Processed;spatialmeasurements

• Trypanosoma brucei

• Axoneme
• Axoneme part
• Cell by organism
• Cortical microtubule, transverse to long axis
• Eukaryotic cell
• Eukaryotic Protist
• Flagellated Protist
• Microtubule basal body

• Ciliary or flagellar motility
• Cortical microtubule organization
• Developing basal body
• Flagellar axoneme assembly

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12595

Doi: https://doi.org/10.6075/J0QV3KMT

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12595

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2022-08-12