Morselli, Eugenia; Mariño, Guillermo; Bennetzen, Martin v.; Eisenberg, Tobias; Megalou, Evgenia; Schroeder, Sabrina; Cabrera, Sandra; Bénit, Paule; Rustin, Pierre; Criollo, Alfredo; Kepp, Oliver; Galluzzi, Lorenzo; Shen, Shensi; Malik, Shoaib Ahmad; Maiuri, Maria Chiara; Horio, Yoshiyuki (2021). CIL:13912, Homo sapiens, permanent cell line cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0XG9PVJ

Human colon carcinoma HCT 116 cells were transfected with a GFP-LC3-encoding plasmid, cultured in complete medium for 24 h and enucleated to obtain cytoplasts. The cytoplasts were treated with 100 µM Resveratrol for 4 h. Cytoplasts were still able to accumulate GFP-LC3 puncta in response to spermidine or resveratrol treatment, indicating that nuclei (and by extension transcription) are not required for short-term autophagy stimulation by these two agents. Nuclei were counterstained by Hoechst. Confocal fluorescent images were captured using a confocal fluorescence microscope (TCS SP2; Leica) fitted with an Apochromat 63× 1.3 NA immersion objective. Images were acquired with a camera (DFC 350 FX 1.8.0; Leica) using LAS AF software (Leica) and processed with Photoshop (CS2; Adobe) software. Specifically, picture processing involved cropping of representative areas and linear adjustments of contrast and brightness and was performed using Photoshop (with equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 8A, middle panel, in Morselli et al. J Cell Biol 192: 615-629

• 2021

• Bennetzen, Martin v.
• Bénit, Paule
• Cabrera, Sandra
• Criollo, Alfredo
• Eisenberg, Tobias
• Galluzzi, Lorenzo
• Horio, Yoshiyuki
• Kepp, Oliver
• Maiuri, Maria Chiara
• Malik, Shoaib Ahmad
• Mariño, Guillermo
• Megalou, Evgenia
• Morselli, Eugenia
• Rustin, Pierre
• Schroeder, Sabrina
• Shen, Shensi

Preparation: isolated subcellular component
Relation to intact cell: enucleated cell
Item type: recorded image; charge coupled device (CCD)
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; differences in adsorption or binding of stain
Visualization methods: EGFP; membrane-permeant probe; Hoechst
Processing history: brightness and contrast adjusted
Data qualification: Processed;intensitiesquantitation

• Cell Image Library Group ID: 5408

• Homo sapiens

• Autophagic vacuole
• Permanent cell line cell

• Autophagy
• HCT 116

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13912

Doi: https://doi.org/10.6075/J0XG9PVJ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13912

Reference

• J Cell Biol 192: 615-629 https://www.ncbi.nlm.nih.gov/pubmed/?term=21339330

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12