Wuehr, Martin; Mitchison, Timothy (2021). CIL:36695, Danio rerio, embryo. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0QR4W2Q

Confocal z-stack of a dividing zebrafish embryo. The fixed embryo is visualized using EMTB-3GFP (microtubules-marker - green), phalloidin (actin marker -red) and Hoechst (DNA marker - blue).

• 2021

• Mitchison, Timothy
• Wuehr, Martin

Images collected on a Zeiss LSM 710 confocal with a 20X 0.8 NA objective using 561, 633, and 750 nm laser lines.

Preparation: methanol fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope; distribution of a specific protein
Visualization methods: labeled primary antibody; phalloidin; hoechst
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Danio rerio

• Actin cytoskeleton
• DNA
• Embryo
• Microtubule

• Mitosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36695

Doi: https://doi.org/10.6075/J0QR4W2Q

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36695

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2025-01-30