Withers, Ginger; Brandner, Dieter (2021). CIL:6134, Rattus, multipolar neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0Z31ZNN

Synapse formation in cultured hippocampal neurons after 7 days in vitro. Cells were immunostained for MAP2, a microtubule associated protein localized to dendrites but not axons (green), and synapsin I, a presynaptic vesicle protein (red) to reveal the development of presynaptic contacts along the dendritic arbor.

• 2021

• Brandner, Dieter
• Withers, Ginger

Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained MAP2 (HM2, from Sigma with d549 conjugated secondary, excitation, 555, emission, 568, Jackson Immunoresearch). Images were acquired with a Leica DMRA microscope with a 16X PL Fluotar (0.5 NA) lens, Photometrics CoolSnap ES CCD camera and MetaMorph software. This merged image was generated using the color combine function in Metamorph.

Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data
Data qualification: Raw

• Cell Image Library Group ID: 9914

• Rattus

• Cytoskeleton
• Dendrite
• Microtubule cytoskeleton
• Multipolar neuron
• Synapse part
• Synaptic vesicle

• Dendrite development
• Establishment of cell polarity
• Neuron development
• Protein localization to synapse

• data
• image

• No linguistic content; Not applicable

Samplenumber: 6134

Doi: https://doi.org/10.6075/J0Z31ZNN

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL6134

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2022-08-12