Valerio-Santiago, Mauricio; Monje-Casas, Fernando (2021). CIL:13874, Saccharomyces cerevisiae. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0668BW6

Tem1 (green) constitutively targeted to spindle pole bodies in nud1-2 cells at the restrictive temperature still displayed problems positioning the spindle (this image shows a rebudded cell, CIL #13873 shows an aligned spindle and CIL# 13889 shows a misaligned spindle) typical of nud1-2, despite the fact that viability was restored. Nud1 is required for mitotic exit and constitutive targeting of Tem1 recovered the mitotic exit defect of nud1-2 cells. DAPI (blue) and differential interference contrast (DIC) image are also shown. Image is Fig 7F, bottom panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 7 include CIL #13888, 13872, 13813, 13889, 13874.

• 2021

• Monje-Casas, Fernando
• Valerio-Santiago, Mauricio

Cells (MATa nud1::kanMX4 leu2::nud1-2::LEU2 pRS316::eGFP-CNM67–TEM1) grown at restrictive temperature 37C were fixed in 2.5% formaldehyde for 10 min, washed twice, and resuspended in 0.1 M potassium phosphate buffer, pH 6.4. Cells were then fixed for 10 min in 80% ethanol and resuspended in 1 mg/ml DAPI. Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x/1.40 NA oil immersion objective lens, A4, L5, and TX2 filters, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (Leica) and ImageJ software.

Preparation: formaldehyde fixed tissue; ethanol fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: widefield illumination; fluorescence microscopy; differential interference contrast microscopy
Parameter imaged: fluorescence emission; optical path length gradient
Source of contrast: distribution of a specific protein; boundaries between regions with different refractive index; compartmentalization of stain or label
Visualization methods: EGFP; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 9034

• Saccharomyces cerevisiae

• Cytoplasm
• Cytoskeleton
• Nuclear envelope
• Nucleus
• Spindle pole body

• Cell cycle
• Cell division
• Exit from mitosis
• GTPase activator activity
• GTPase activity
• Mitosis
• Mitotic cell cycle spindle orientation checkpoint
• Nucleotide binding
• Protein binding
• Regulation of exit from mitosis
• Small GTPase mediated signal transduction
• Structural constituent of cytoskeleton
• W303

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13874

Doi: https://doi.org/10.6075/J0668BW6

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13874

Reference

• J Cell Biol. (2011) 192: 599-614. https://www.ncbi.nlm.nih.gov/pubmed/?term=21321099

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12