CIL:11923, Rattus, neuron soma, CA1 stratum pyramidale of the hippocampus
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Petralia, Ronald S. (2021). CIL:11923, Rattus, neuron soma, CA1 stratum pyramidale of the hippocampus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0G1600D
- Description
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NMDA receptors (NMDARs) can exit the Golgi/TGN via clathrin-coated vesicles. Double-labeling using rabbit polyclonal antibodies to AMPA receptors (AMPARs) (combination of 3 antibodies: GluR1, GluR2, GluR2/3) and 10 nm immunogold, and mouse monoclonal antibodies to the NMDAR subunit NR1 (3 antibodies) and 5 nm immunogold, in a section of a neuron soma in the CA1 stratum pyramidale of the hippocampus from a P10 rat. In these studies, identification of 5 nm gold particles, where unclear, was confirmed by detailed examination and photography at higher magnifications. In the second image in the series, the micrograph is repeated and major structures are highlighted in color (green for membranous structures and clathrin coats; red for AMPAR-10 nm gold; blue for NR1-5 nm gold). Note that AMPA and NMDA receptor labeling is localized mainly separately in both the Golgi apparatus and trans-Golgi network (TGN), and NR1 is found alone in several clathrin-coated vesicles (CCVs) budding off of the TGN. Image is Fig1 from Wenthold, R.J., R.A. Al-Hallaq, C.C. Swanwick, and R.S. Petralia (2008) Molecular properties and cell biology of the NMDA receptor. In Structural and Functional Organization of the Synapse (J.W. Hell and M.E. Ehlers, eds.). Springer, NY, pp. 317-367.
- Date Issued
- 2021
- Researcher
- Technical Details
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Preparation: chemically fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image; graphic illustration
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein
Visualization methods: gold
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements - Scientific Name
- Anatomy
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- Identifier
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Samplenumber: 11923
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11923
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12