CIL:48106, Rattus norvegicus, fetal cardiomyocyte
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- Collection
- Cite This Work
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Prigozhina, Natalie (2021). CIL:48106, Rattus norvegicus, fetal cardiomyocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0VT1S8Z
- Description
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Rat cardiomyocytes were plated and stained for actin (in red), desmin (in green) to reveal the intermediate filament essential for the structural integrity and function of muscle, and DAPI (in blue) to label nuclei.
- Date Issued
- 2021
- Researcher
- Methods
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Plated rat cardiomyocytes were fixed and immunostained for actin (from Santa Cruz), desmin (from Sigma), and DAPI (from Life Technologies). Images were acquired with a 40X (Plan Fluor, NA 0.75) lens, SPOT RT Slider CCD camera and SPOT Basic image capture software. Image generated with the Image J color combine function.
- Technical Details
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Relation to intact cell: dispersed cells in vitro
Item type: charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody
Data qualification: Processed - Scientific Name
- Anatomy
Formats
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 48106
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL48106
Source data
- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12