Microscopy product ID: 4097
Segmentation
Description | Manual segmentation of the individual cristae and inner and outer mitochondrial membranes using Xvoxtrace 2.18. Contours were traced every fourth section starting on plane 4 |
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Description | Zip file (RudyE_seg.zip) containing the segmentation file generated by Xvoxtrace and converted into Jinx format (RudyE_seg.jnx), the subvolume used for tracing in Analyze 7.5 format (RudyE_scirun.img/.hdr) and the surfaced objects in Synu format (*.synu). Note: For unknown reasons, the .trace file originally generated by Xvoxtrace could not be uploaded. The Analyze 7.5 volumes were also acting a bit strange, but could be uploaded after regenerating them using ImageJ. Both the segmentation and volume files could be opened in Jinx, and so the manual segmentations were saved as a Jinx file. Jinx can be downloaded from the CCDB tool web page. |
Reconstruction
Description | Computed slice through a tomographic volume of isolated mitochondria from mouse liver after treatment with the BH3 peptide domain from Bid. |
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Description | Zip file (rudyE_vol.zip) containing the reconstruction file in Analyze 7.5 format (RudyEsmall.img/hdr). Note: This volume was likely downsampled from the original volume and thus the stated voxel size is likely incorrect. The full sized volume file could not be located. Also note, a subvolume was generated from this reconstruction for segmentation (RudyE_scirun.img/hdr). The subvolume used for tracing may be downloaded along with the other segmentation files. The location of the subvolume can be viewed in the image map stored with this record. |
Animation
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Description | Animation through a portion of the tomographic volume of isolated mitochondria treated with the BH3 peptide domain from Bid. The volume was downsampled for ease of viewing. |
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Yamaguchi, Ryuji; Newmeyer, Don; Lartigue, Lydia; Perkins, Guy A.; Scott, Ray T.; Dixit, Amruta; (2017). Microscopy product ID: 4097. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0DZ083G
- Date Issued
- 2017
- Research Team Head
- Researchers
- Technical Details
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Male
Product type: SINGLE TILT. Microscopy type: IVEM. Instrument: JEOL 4000EX IVEM
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- Scientific Name
- Anatomy
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- No linguistic content; Not applicable
- Related Resources
- Yamaguchi R, Lartigue L, Perkins G, Scott RT, Dixit A, Kushnareva Y, Kuwana T, Ellisman MH, Newmeyer DD (2008). Opa1-mediated cristae opening is Bax/Bak and BH3 dependent, required for apoptosis, and independent of Bak oligomerization. Molecular Cell, 31(4):557-569. PubMed: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2636708; Molecular Cell. https://doi.org/10.1016/j.molcel.2008.07.010
- Microscopy product 4097 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB4097
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Creative Commons Attribution 4.0 International Public License
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- UC Regents
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Under copyright (US)
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22