{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1595991277},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":true},"CIL":{"CORE":{"TECHNICALDETAILS":{"free_text":"populations of ~1200 cells plated on glass in standard media were imaged at 37º C in each experiment.  A target subpopulation of ~100 cells display a readily observed extended, ruffling leading edge pseudopod .  The cells exhibiting these deployed, extended pseudopods were counted in each unit area at 6 timepoints during the 3 hr observation period following rapid addition of drug to its standard working concentration.  Altogether, 6 experiments were carried out for each treatment, yielding average timecourses summarizing observations of ~600 polarized cells.<br\/><br\/>\r\n<b>Images are provided as uncorrected, 16 bit .nd2 files to preserve all original data.  To view these .nd2 files:<\/b>\r\n<br\/>1. Download ImageJ viewer at (https:\/\/imagej.nih.gov\/ij\/download.html)\r\n<br\/>2. Download the BioFormats.jar package at (https:\/\/www.openmicroscopy.org\/bio-formats\/downloads\/)\r\n<br\/>3. Open the ImageJ directory after installation and drag the extracted BioFormats.jar package into the \"Plugins\" subdirectory \r\n<br\/>4. Open an .nd2 image using imageJ and select opening options when prompted, if desired. If no modifications are desired, select \"OK\"\r\n<br\/>5. The 16 bit image will typically look dark and will require brightness adjustment.\r\n<br\/>6. In the ImageJ toolbar, select Image >> Adjust >> Brightness\/Contrast and select \"Auto\", or manually set the brightness and contrast."},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"ATTRIBUTION":{"OTHER":["W9CILP20451","Individual_Timepoints","Acetaminophen","171012"],"Contributors":["Thomas Buckles"]},"IMAGINGMODE":[{"free_text":"DICM"}],"BIOLOGICALPROCESS":[{"free_text":"Cell polarity establishment & maitenance "}],"IMAGEDESCRIPTION":{"free_text":"A novel cell population analysis to quantify both initial and long-term drug effects, namely pseudopod adaptation and recovery following an initial drug-induced perturbation, adding a full therapeutic dose at once at t=0."},"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"}],"CELLULARCOMPONENT":[{"free_text":"whole cell"}],"VISUALIZATIONMETHODS":[{"free_text":"Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective"}],"GROUP_ID":"20031","CELLTYPE":[{"free_text":"RAW 264.7 TIB-71"}]},"Image_files":[{"File_type":"Jpeg","File_path":"50956.jpg","Size":150920,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"50956.zip","Size":115536595,"Mime_type":"application\/zip"}]},"Citation":{"Title":"Thomas Buckles (2020) CIL:50956, Mus musculus, RAW 264.7 TIB-71. CIL. Dataset. CIL. Dataset","ARK":"ark:\/b7295\/w9cil50956","DOI":"doi:10.7295\/W9CIL50956"}}}