{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1315022400},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"37412.jpg","Size":31855,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"37412.zip","Size":2883712,"Mime_type":"application\/zip"},{"File_type":"Mp4","File_path":"37412_web.mp4","Size":1152818,"Mime_type":"video\/mp4"}],"CORE":{"ATTRIBUTION":{"OTHER":["Zebrafish - The Living Laboratory"],"URLs":[{"Label":"Fishscope","Href":"http:\/\/depts.washington.edu\/fishscop\/Boss\/pages\/movie_pages\/landmarks\/landmarks.html"}],"Contributors":["Mark Cooper"]},"ZEBRAFISH_ANATOMY":[{"onto_name":"hypoblast","onto_id":"ZFA:0000117"},{"onto_name":"notochord","onto_id":"ZFA:0000135"},{"onto_name":"neural plate","onto_id":"ZFA:0000132"},{"onto_name":"neural keel","onto_id":"ZFA:0000131"},{"onto_name":"floor plate","onto_id":"ZFA:0000022"},{"onto_name":"hindbrain","onto_id":"ZFA:0000029"},{"onto_name":"neuromere","onto_id":"ZFA:0001328"},{"onto_name":"otic placode","onto_id":"ZFA:0000138"}],"BIOLOGICALPROCESS":[{"onto_name":"anatomical structure morphogenesis","onto_id":"GO:0009653"},{"free_text":"intercalation"},{"free_text":"mediolateral convergence"},{"onto_name":"cell division","onto_id":"GO:0051301"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"Embryonic cell","onto_id":"FMA:82840"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Zebrafish Embryo, from tailbud to 16-hour stage, stained with 100 micromolar Bodipy-Ceramide. Bodipy-Ceramide permeates the enveloping layer epithelium of the embryo, accumulating in the interstitial spaces of the segmentation cavity. The sequence begins at the level of the hypoblast. Chorda mesoderm cells intercalate in a mediolateral fasion. This cellular convergence causes the chorda mesoderm to extend in an axial direction. During cell intercalation, the notochord develops a transient bilateral symmetry. As the sequence progresses, the notochord is depressed ventrally by mediolateral convergence of cells in the neural plate (this begins neural keel formation). The floorplate comes into view. Cells at the level of the floorplate along the embryonic midline begin to round up in preparation for mitosis. At one point at least four cells are undergoing synchronous division. The daughter cells of these divisions segregate to either side of the embryonic midline. The embryo undergoes a torsional rotation. The rotation brings into view the edge of the neural keel at the level of the hindbrain. The ridges along the edge of the neural keel delineate neuromeres of the hindbrain. The sequence ends with a view of the otic placode. The time-lapse sequence compresses approximately 11 hours of development. This video made available by Mark Cooper thru Zebrafish - The Living Laboratory. The videos in this group can also be found in the Developmental Landmarks page of the Fishscope web site."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"free_text":"interstitial space"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"},"GROUP_ID":"9332","DATAQUALIFICATION":{"free_text":"RAW"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"DIMENSION":[{"Space":{"axis":"X"}},{"Space":{"axis":"Y"}}],"SPECIESTAXASPECIFIC":[{"onto_id":"ZFA:ZFA:0000117"},{"onto_id":"ZFA:ZFA:0000135"},{"onto_id":"ZFA:ZFA:0000132"},{"onto_id":"ZFA:ZFA:0000131"},{"onto_id":"ZFA:ZFA:0000022"},{"onto_id":"ZFA:ZFA:0000029"},{"onto_id":"ZFA:ZFA:0001328"},{"onto_id":"ZFA:ZFA:0000138"}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Danio rerio","onto_id":"NCBITaxon:7955"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":[{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},{"free_text":"bodipy ceramide"}],"CELLTYPE":[{"onto_name":"epithelial cell","onto_id":"CL:0000066"},{"free_text":"chorda mesoderm"}]}},"Citation":{"Title":"Mark Cooper (2011) CIL:37412, Danio rerio, epithelial cell, chorda mesoderm. CIL. Dataset","ARK":"ark:\/b7295\/w9cil37412","DOI":"doi:10.7295\/W9CIL37412"}}}